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Biocompatibility / Safety Testing for Medical Devices  

 

For a table that outlines the ISO/FDA test modalities frequently used to study the biological safety and biocompatibility of medical devices, click here 

 

These tests should be conducted on final product or representative samples according to the category of the device (based on anatomic location and duration of contact).  AppTec's comprehensive menu of services includes all the tests related to the table’s “Tests for Consideration,” as well as those tests that may be required for Japanese (JMHLW) submissions. 

 

For regulatory clearance purposes, most tests should be carried out under controlled laboratory conditions in compliance with Good Laboratory Practices (GLP). 

 

AppTec's biocompatibility / safety testing services feature:

• Dedicated isolated study suites

• Interpretation of histopathology and other toxicology data

• Regulatory consultation to optimize study for specific devices 

 

AVAILABLE TESTING SERVICES INCLUDE: 

Cytotoxicity (in vitro)

Agarose Overlay (USP, ISO)

MEM Elution (USP, ISO)

Direct Contact

Growth InhibitionExtract Colony Assay (JMHLW) 

 

Sensitization (in vivo)

Guinea Pig Maximization

Guinea Pig Repeated Patch (Buehler)

Murine Local Lymph Node Assay (LLNA)

Maximization Sensitization Test (JMHLW)    

 

Irritation/Intracutaneous Reactivity (in vivo)

Intracutaneous (USP, ISO, JMHLW)

Primary Skin Irritation (ISO, JMHLW)

Ocular Irritation

Intraocular Irritation

Mucosal Irritation:  Oral • Vaginal • Bladder

Acute Dermal Limit 

 

Systemic (Acute) Toxicity (in vivo)

Acute Systemic Toxicity (USP, ISO, JMHLW)

Pyrogen–Materials Mediated (USP, ISO, JMHLW)

Mouse Safety & Abnormal Toxicity  

 

Subacute/Subchronic Toxicity (in vivo)

Subacute Toxicity – Intravenous • Intraperitoneal

Subchronic Toxicity (JMHLW)  

 

Genotoxicity (in vitro and in vivo)

In vitro Bacterial Mutagenicity (Ames)

In vitro Mouse Lymphoma

In vitro Chromosomal Aberration

In vivo Mouse Micronucleus

Bacterial Reverse Mutation (JMHLW) 

In vitro Chromosome Aberration (JMHLW) 

 

Implantation (in vivo)

Intramuscular Implant (USP, ISO, JMHLW)

Subcutaneous Implant

Intraperitoneal Implant 

 

Hemocompatibility (in vitro and in vivo)

Hemolysis Test

      – Extract Method (ASTM or NIH)

      – Direct Contact Method (ASTM or NIH)

      – Hemolytic Toxicity (JMHLW)

Complement Activation

Coagulation Studies

Platelet and Leukocyte Counts

Intravascular Thrombogenicity  

 

Chronic Toxicity

Subcutaneous • Intraperitoneal

Dermal • Intravenous • Oral   

 

Carcinogenicity Clinical Pathology

Chemistry – 18 Test Parameters

Hematology – 14 Test Parameters 

 

Histopathology / Immunochemistry Other Biocompatibility Testing

USP Rabbit Pyrogen Test

LAL Bacterial Endotoxin Tests for Pyrogenicity

USP Safety Test in Mice and Guinea Pigs

In vivo Assay for Viral Contaminants

Cytotoxicity Screening of Dissolvable Materials

USP Class I - VI 

 

Part 18 Risk Assessment and Analytical Chemistry 

 

Customized assays, complete toxicology and custom implant studies also available.