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Flow Cytometry Assays

 

 

AppTec offers a variety of flow cytometry-based assays in support of process development, lot release qualification, or stability programs.  These assays are performed under GLP/GMP conditions designed to meet the needs of the cell therapy and biopharmaceutical industries. 

 

Assays and studies can be executed per specific instruction, performed using standard protocols, or developed “from the ground up,” based on guidelines and project goals. Our experienced scientists and supporting departments work with the Sponsor to ensure efficient transfer of study-related materials, as well as design of optimization studies, operating procedures and results analysis. 

 

 

  

Flow cytometry assays available from AppTec include:

 

  Antigen expression and stability

 

  Activation/Suppression studies

 

  Apoptosis

 

  Cell cycle analysis

 

  Gene expression/transfection studies (e.g., GFP expression)

 

  Cytokine production

 

  Titration and cross-blocking studies

 

  QC of manufactured antibodies and/or fluorescent conjugates

 

  Antibody panel screening for rule-out/rule-in of candidates

 

  Assay development and other custom services available upon request